Jepson Bolton's International Catalogue
Hybridisation Ovens and Incubators
Hybridisation Incubator Stuart
Conform to IEC 1010
Available in two sizes, these incubators have stainless steel interiors with fan circulation and digital display electronic temperature control with platinum resistance sensor, ensuring high sensitivity and fast response. The ovens have efficient insulation to minimise heat losses and provide fast recovery and a cool outer case. A toughened glass inner door is titted together with adjustable vent and ladder rack shelf support system. The units can be used as conventional incubators as well for hybridisation processes.
The smaller (50 litre) model will accept one removable bottle rotisserie with a capacity of 12 bottles and a rotation speed of 6 5 rpm. An alternative version of this is additionally equipped with a linear shaker located on the base of the incubator. The shaker has a variable shaking speed of 10 to 70 stokes per minute and the platform is removeable Rotisserie and shaker have separate switches on the control panel
The larger (100 litre) model accepts two of the 12-bottle rotisseries and again a version is available with additional linear shaker. In this model it is possible to operate one rotisserie plus the shaker and have a shelf available for simultaneous storage and incubation
Model type S101 S102 Capacity ltrs 50 100 Temperature range °C 30 to 100 30 to 100 Temperature fluctuation°C +/-0.1 +/-0.1 Temperature variation °C +/-0.25 +/-0.5 Rotisserie rotation rpm 6.5 6.5 Rotisserie bottle capacity 12 12 Shaker speed strokes/min 10 to 70 10 to 70 Dimensions: internal l x w x h mm 340 x 390 x 395 400 x 390 x 675 external l x w x h mm 460 x 690 x 540 510 x 690 x 825 Heater load W 400 750 Electrical requirements 240V 50 H both models
M67-010 Hybridisation incubator 50 ltr Stuart S101H complete with one rotisserie and drip tray £1760.00 M67-012 Hybridisation incubator 50 ltr Stuart S101HS complete with one rotisserie, linear shaker and drip tray £2035.00 M67-014 Hybridisation incubator 100 ltr Stuart S102H complete with two rotisseries and drip tray £2200.00 M67-016 Hybridisation incubator 100 ltr Stuart S102HS complete with two rotisseries, linear shaker, one shelf and drip tray £2475.00 M67-020 Spare stainless steel drip tray for 50 ltr models £42.00 M67-022 Spare stainless steel drip tray for 100 ltr models 46.00 M67-026 Rotisserie for 12x35mm dia bottles £201.00 M67-028 Rotisserie for 8 x 70mm dia bottles £193.00 M67-030 Stand, white acetal, for rotisseries (M67-026/028) £40.00
Hybridisation Oven/shaker StuartDual purpose unit which may be configured either as a hybridisation incubator with bottle rotisserie or as a rocking shaker. The oven is supplied complete with a bottle rotisserie which accepts six hybridisation bottles around the periphery and a further bottle in the centre for buffer solution. The rotisserie rotates on rollers and the speed is variable between 2 and 10rpm. The function of the incubator is easily changed by removing the rotisserie and lowering a platform at the rear of the interior to form a rocking shaker with a variable speed of 5 to 70 oscillations per minute. The unit is of robust construction with stainless steel interior and upwards-opening door with double glazed panel of acrylic and polycarbonate which withstands temperatures up to 80°C and provides protection from li-emitting isotopes.
Temperature is controlled by means of a platinum resistance sensor and electronic controller with digital temperature indicator and this, together with forced air circulation, provides a precisely controlled internal temperature. The front panel has controls for On/Off, temperature setting and display with LCD readout and over-temperature selection with fault indication. The oven is supplied with a rotisserie for 7 bottles (I x dia 230 x 35mm) and a shaking platform with variable speed 5 to 70 osc/min. A rotisserie cradle for two larger bottles (I x dia 230 x 70mm) is also available.
Capacity 20 ltrs Temperature range Ambient +5 to 80°C Temperature control accuracy +/-0.25°C Temperature fluctuation +/-0.1°C Rotisserie speed 2 to 10 rpm Rocking shaker speed 5 to 70 osc/min Angle of tilt 4°, 7° or 10° selectable Dimensions internal without platform l x w x h 250 x 285 x 240 mm with platform l x w x h 230 x 250 x 200 mm Dimensions external l x w x h 350 x 360 x 440 mm Electrical requirements 240V 50Hz 250W
M67-040 Hybridisation oven/shaker Stuart model Sl20H with rotisserie for 7 bottles and rockinq platform £1600.00 M67-042 Rotisserie cradle, accepts 2 bottles l x dia 230 x 70mm £136.00 M67-060 Hybridisation bottle l x dia 230 x 35mm £26.00 M67-062 Hybridisation bottle l x dia 230 x 70mm £48.50
Hybridisation bottles StuartFour sizes of hybridisation bottle are available for use with the Stuart Hybridisation Incubators (M67-010/016 and M67-040) The bottles are manufactured from heavy wall borosilicate glass with rounded bottom and flame-polished top lip. The blue caps are suitable for autoclaving and can withstand temperatures up to 140C.
M67-060 Hybridisation bottle l x dia 230 x 35 mm £28.00 M67-062 Hybridisation bottle l x dia 230 x 70 mm £52.00 M67-064 Hybridisation bottle l x dia 300 x 35 mm £35.00 M67-066 Hybridisation bottle l x dia 150 x 35 mm £28.00
Microplate Incubator Stuat Sl 18Microtitration plates are difficult to incubate as they are flat and solid. If more than three or four are placed on a shelf in a conventional incubator they block the air circulation which dramatically affects the temperature variation and fluctuation in the chamber. The Stuart Sl 18 incubator is designed so that preheated air is blown from the left hand side across each shelf, rather than 'through' each shelf, and then taken in through the right hand side for recirculation. A further problem common with the incubation of microtitre plates is that drying occurs the Sl 18 has the provision of a water tray to increase humidity within the chamber and minimise this effect.
To accommodate the maximum number of samples, shelves are positioned close together and captive, but may be slid out to a retaining stop to facilitate loading. Each shelf can accommodate six microtitre plates, or twelve plates double stacked. The door is manufactured from 1 Omm thick acrylic sheet and is upward hinged to minimise the use of bench space and allow right or left handed operation.
Temperature range 30 to 60°C Temperature fluctuation +/-O.1°C* No of shelves supplied 4 No of trays supplied Dimensions: internal l x w x h mm 260 x 285 x 245 mm external l x w x h mm 370 x 365 x 440 mm Weight 20kg Heater load 250 W Electrical requirements 240V 50Hz
*at 37°C loaded with 24 microplates
M67-080 - Microtitre plate incubator Stuart SI 18 - £904.00
Hybridisation Incubator Techne HB-lDAn advanced hybridisation incubator for DNA and other probes. Blotting techniques in which DNA RNA or protein are immobilised on to nylon or nitrocellulose membranes, form a cornerstone of molecular biology Detection of the desired sequence is usually performed using radioactive probes. Given the expense and hazardous nature of the probes, probing should.
a) be performed under safe conditions
b) use minimal volumes (to improve signal and reduce expense)
c) allow re-use of materials
d) be simple to use
The Techne Hybridiser meets all these requirements and replaces the cumbersome shaker bath and plastic bag method. At least 20 x 20cm membranes can be probed in the large hybridisation tube in DNA experiments. Hybridisations can be performed in a minimal volume of 5ml, an 80% reduction over conventional systems, reducing expense and improving signal. The operator is protected from irradiation throughout and the apparatus is extremely simple to use. The capacity to run up to five membranes in each large hybridisation tube without any increase in backgund means that a large number of high quality hybridisations can be performed simultaneously. The unit accepts up to six large or small hybridisation tubes or 24 mini tubes.
The incubator has digital set and display of temperature and is microprocessor controlled. A glass door allows viewing of the samples and the incubator is provided with levelling feet. Separation membranes are also available .
The HB-1D is supplied complete with three large hybridisation tubes. Hybridisation Incubator Techne continued
Temperature range 5°C above ambient to 100°C Temperature stability: in the oven >1°C in tubes >0 25°C Temperature uniformity: in the oven better than +/-3°C in tubes +/-1.5°C Set point resolution 1°C Absolute accuracy +/-2°C Warm-up time (within tube) 50 mins Speed of rotation 4 rpm No of tubes max 6 large/small or 24 mini No of membranes per tube at least 5 (large tube) Probe volume as little as 5ml required Dimensions internal l x w x h 210 x 260 x 330 mm Dimensions external l x w x h 285 x 385 x 550 mm Weight 20 kg Heater load 750W Electrical requirements 240V 50Hz
M67-100 Hybridisation incubator Techne HB-1D £1868.00
M67-110 Large glass tube and end caps £80.00 M67-112 Small glass tube with screw cap £58.00 M67-114 Multi tube holder for mini tubes £92.00 M67-116 Mini tubes. Pack of 4 £80.00 M67-118 Tube rack holder (holds 3 large tubes) £33.00 M67-120 Separation membranes 20 x 20cm. Pack of 5 £48.00
Hybridisation Incubator Techne HB-2DThis compact bench or wall mounted hybridisation incubator has the capability of holding one large (80mm) hybridisation tube, three 44mm tubes or four 32mm tubes. The unit has analogue set and digital display of temperature. The HB-2D is supplied complete with one large hybridisation tube.
Temperature range 5°C above ambient to 100°C Temperature uniformity: in tubes +/-1·0°C Control precision +/-1°C Warm-up time (within tube) 50 mins Speed of rotation 10 rpm No of tubes max 1 large, 4 mini, 1 small or 3 small-plus No of membranes per tube at least 5 (large tube) Probe volume as little as 5ml required Dimensions internal l x w x h 108 x 305 x 110 mm Dimensions external l x w x h 230 x 360 x 270 mm Weight 5 kg Heater load 750W Electrical requirements 240V 50Hz
M67-125 - Hybridisation incubator Techne HB-2D £1070.00
M67-131 Large glass tube and end caps £85.00 M67-133 Small glass tube with screw cap £58.00 M67-135 Multi tube holder for mini tubes £92·00 M67-136 Mini tubes. Pack of 4 £80.00 M67-138 Tube holder for small-plus tubes £92.00 M67-140 Small-plus tube £58.00 M67-142 Small-plus 3-tube assembly with 3 tubes £269.00 M67-120 Separation membranes 20 x 20cm. Pack of 5 £48.00
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